time 0μM 1.875μM 3.75μM 7.5μM 15μM 30μM 60μM 120μM 240μM 480μM 1.5 0.437 0.445 0.437 0.441 0.457 0.466 0.468 0.467 0.459 0.483 3.0 0.434 0.443 0.437 0.437 0.445 0.456 0.466 0.469 0.462 0.480 4.5 0.440 0.447 0.439 0.441 0.449 0.456 0.465 0.474 0.466 0.476 6.0 0.440 0.448 0.442 0.440 0.449 0.452 0.466 0.474 0.470 0.487 7.5 0.444 0.453 0.447 0.447 0.454 0.458 0.471 0.480 0.475 0.482 9.0 0.448 0.457 0.450 0.449 0.457 0.463 0.476 0.484 0.477 0.481 10.5 0.458 0.464 0.456 0.454 0.466 0.467 0.481 0.491 0.484 0.503 12.0 0.460 0.468 0.460 0.460 0.469 0.472 0.484 0.495 0.489 0.497 13.5 0.463 0.473 0.464 0.466 0.475 0.479 0.491 0.496 0.489 0.500 15.0 0.467 0.478 0.467 0.469 0.476 0.480 0.490 0.505 0.495 0.512 16.5 0.470 0.481 0.474 0.475 0.482 0.484 0.498 0.507 0.499 0.506 18.0 0.476 0.486 0.479 0.478 0.486 0.492 0.506 0.513 0.505 0.528 19.5 0.480 0.488 0.480 0.481 0.492 0.499 0.515 0.519 0.509 0.519 21.0 0.485 0.493 0.487 0.485 0.496 0.500 0.514 0.524 0.511 0.520 22.5 0.486 0.498 0.490 0.490 0.501 0.507 0.521 0.530 0.516 0.537 24.0 0.492 0.504 0.493 0.494 0.505 0.511 0.528 0.533 0.520 0.544 25.5 0.496 0.505 0.496 0.498 0.509 0.517 0.525 0.536 0.521 0.544 27.0 0.498 0.511 0.501 0.498 0.513 0.519 0.531 0.541 0.528 0.534 28.5 0.500 0.510 0.503 0.506 0.515 0.523 0.535 0.542 0.526 0.546 30.0 0.504 0.514 0.507 0.508 0.516 0.525 0.537 0.545 0.532 0.538 31.5 0.509 0.520 0.515 0.513 0.524 0.531 0.541 0.549 0.535 0.554 33.0 0.514 0.524 0.515 0.518 0.526 0.531 0.545 0.551 0.537 0.558 34.5 0.518 0.526 0.517 0.522 0.532 0.538 0.550 0.554 0.541 0.550 36.0 0.521 0.532 0.524 0.524 0.538 0.541 0.555 0.560 0.542 0.550 37.5 0.525 0.534 0.531 0.532 0.542 0.545 0.556 0.563 0.547 0.557 39.0 0.528 0.539 0.532 0.533 0.544 0.548 0.559 0.566 0.548 0.561 40.5 0.534 0.545 0.535 0.536 0.545 0.551 0.558 0.568 0.552 0.572 42.0 0.538 0.548 0.542 0.540 0.553 0.559 0.570 0.574 0.555 0.561 43.5 0.542 0.550 0.548 0.547 0.558 0.563 0.572 0.573 0.556 0.569 45.0 0.548 0.557 0.551 0.553 0.562 0.566 0.575 0.579 0.559 0.570 46.5 0.550 0.562 0.554 0.556 0.567 0.572 0.583 0.584 0.562 0.577 48.0 0.558 0.563 0.560 0.560 0.574 0.577 0.585 0.588 0.560 0.572 49.5 0.561 0.571 0.565 0.570 0.581 0.586 0.592 0.590 0.568 0.575 51.0 0.567 0.576 0.569 0.571 0.583 0.587 0.592 0.594 0.567 0.578 52.5 0.570 0.580 0.574 0.575 0.588 0.593 0.601 0.597 0.570 0.579 54.0 0.578 0.586 0.580 0.585 0.592 0.597 0.607 0.601 0.575 0.586 55.5 0.582 0.592 0.590 0.588 0.600 0.603 0.609 0.603 0.575 0.581 57.0 0.589 0.600 0.592 0.595 0.605 0.614 0.614 0.609 0.581 0.585 58.5 0.596 0.606 0.600 0.599 0.612 0.614 0.620 0.614 0.582 0.587 60.0 0.600 0.612 0.605 0.606 0.617 0.623 0.628 0.618 0.583 0.582 61.5 0.610 0.615 0.610 0.609 0.620 0.625 0.621 0.623 0.583 0.575 63.0 0.614 0.624 0.618 0.620 0.630 0.633 0.636 0.626 0.588 0.578 64.5 0.619 0.629 0.623 0.623 0.636 0.639 0.641 0.629 0.590 0.575 66.0 0.627 0.638 0.631 0.634 0.645 0.647 0.648 0.638 0.593 0.579 67.5 0.634 0.644 0.634 0.638 0.649 0.650 0.650 0.639 0.592 0.586 69.0 0.642 0.651 0.645 0.648 0.658 0.660 0.658 0.640 0.599 0.587 70.5 0.649 0.659 0.654 0.655 0.663 0.667 0.665 0.649 0.598 0.589 72.0 0.653 0.668 0.660 0.661 0.671 0.675 0.672 0.652 0.602 0.583 73.5 0.662 0.672 0.667 0.666 0.675 0.680 0.675 0.655 0.604 0.577 75.0 0.670 0.680 0.671 0.673 0.684 0.690 0.685 0.661 0.606 0.581 76.5 0.676 0.686 0.679 0.684 0.691 0.694 0.692 0.667 0.608 0.588 78.0 0.681 0.693 0.683 0.689 0.701 0.704 0.698 0.673 0.607 0.582 79.5 0.691 0.701 0.691 0.696 0.706 0.712 0.703 0.679 0.613 0.587 81.0 0.697 0.709 0.700 0.704 0.713 0.719 0.709 0.685 0.616 0.584 82.5 0.702 0.713 0.706 0.709 0.720 0.725 0.718 0.687 0.616 0.575 84.0 0.709 0.719 0.713 0.715 0.728 0.731 0.722 0.695 0.620 0.577 85.5 0.718 0.727 0.718 0.721 0.734 0.739 0.731 0.699 0.620 0.594 87.0 0.726 0.734 0.723 0.728 0.741 0.742 0.734 0.707 0.623 0.582 88.5 0.732 0.739 0.734 0.735 0.747 0.750 0.742 0.711 0.625 0.580 90.0 0.736 0.748 0.740 0.744 0.756 0.763 0.752 0.716 0.628 0.588 91.5 0.745 0.754 0.744 0.747 0.758 0.761 0.753 0.720 0.629 0.573 93.0 0.753 0.758 0.754 0.756 0.765 0.771 0.764 0.728 0.633 0.584 94.5 0.758 0.766 0.758 0.764 0.776 0.777 0.768 0.733 0.633 0.591 96.0 0.768 0.775 0.767 0.770 0.780 0.784 0.772 0.737 0.637 0.585 97.5 0.769 0.779 0.773 0.775 0.785 0.792 0.784 0.742 0.638 0.584 99.0 0.778 0.785 0.780 0.781 0.792 0.798 0.786 0.750 0.641 0.583 100.5 0.785 0.793 0.787 0.786 0.797 0.800 0.787 0.754 0.643 0.577 102.0 0.791 0.801 0.793 0.795 0.807 0.812 0.800 0.763 0.644 0.582 103.5 0.796 0.804 0.800 0.804 0.816 0.822 0.810 0.767 0.647 0.584 105.0 0.803 0.812 0.808 0.810 0.819 0.828 0.815 0.772 0.647 0.581 106.5 0.810 0.820 0.814 0.815 0.827 0.833 0.820 0.777 0.650 0.583 108.0 0.821 0.827 0.818 0.821 0.833 0.839 0.823 0.787 0.652 0.581 109.5 0.824 0.832 0.825 0.828 0.842 0.849 0.835 0.791 0.657 0.587 111.0 0.833 0.840 0.833 0.833 0.848 0.849 0.834 0.798 0.657 0.576 112.5 0.840 0.848 0.839 0.841 0.856 0.858 0.841 0.804 0.659 0.582 114.0 0.844 0.852 0.847 0.849 0.862 0.871 0.853 0.807 0.660 0.591 115.5 0.851 0.858 0.854 0.855 0.869 0.875 0.858 0.813 0.665 0.583 117.0 0.861 0.863 0.858 0.861 0.873 0.880 0.863 0.821 0.666 0.584 118.5 0.867 0.871 0.868 0.868 0.881 0.888 0.873 0.828 0.668 0.589 120.0 0.871 0.878 0.875 0.875 0.887 0.895 0.877 0.832 0.668 0.583 121.5 0.877 0.883 0.881 0.882 0.893 0.904 0.883 0.839 0.670 0.588 123.0 0.887 0.892 0.885 0.885 0.899 0.905 0.886 0.842 0.673 0.584 124.5 0.895 0.900 0.893 0.895 0.907 0.913 0.895 0.848 0.676 0.582 126.0 0.902 0.905 0.900 0.899 0.912 0.920 0.902 0.854 0.676 0.579 127.5 0.907 0.913 0.909 0.906 0.920 0.927 0.907 0.858 0.676 0.580 129.0 0.917 0.919 0.915 0.913 0.927 0.933 0.911 0.866 0.678 0.579 130.5 0.921 0.926 0.921 0.919 0.933 0.938 0.916 0.873 0.683 0.585 132.0 0.925 0.931 0.929 0.927 0.940 0.949 0.926 0.875 0.683 0.584 133.5 0.935 0.938 0.935 0.933 0.947 0.953 0.934 0.882 0.686 0.590 135.0 0.941 0.942 0.941 0.940 0.953 0.957 0.939 0.887 0.687 0.589 136.5 0.945 0.948 0.948 0.945 0.957 0.965 0.943 0.894 0.690 0.581 138.0 0.954 0.955 0.951 0.951 0.961 0.965 0.945 0.900 0.693 0.589 139.5 0.960 0.959 0.957 0.953 0.965 0.969 0.947 0.905 0.695 0.586 141.0 0.961 0.961 0.960 0.956 0.967 0.975 0.955 0.908 0.695 0.582 142.5 0.965 0.964 0.962 0.957 0.968 0.970 0.956 0.912 0.696 0.580 144.0 0.970 0.968 0.963 0.961 0.971 0.973 0.953 0.920 0.700 0.579 145.5 0.971 0.969 0.965 0.961 0.973 0.973 0.958 0.921 0.700 0.579 147.0 0.971 0.969 0.966 0.963 0.973 0.979 0.962 0.922 0.705 0.584 148.5 0.976 0.972 0.967 0.963 0.972 0.977 0.961 0.930 0.705 0.587 150.0 0.972 0.969 0.970 0.962 0.975 0.979 0.965 0.933 0.707 0.589 151.5 0.973 0.972 0.971 0.966 0.976 0.981 0.968 0.936 0.711 0.581 153.0 0.975 0.974 0.970 0.963 0.974 0.980 0.962 0.938 0.710 0.575 154.5 0.976 0.974 0.971 0.966 0.977 0.980 0.967 0.939 0.713 0.587 156.0 0.977 0.974 0.973 0.966 0.977 0.981 0.966 0.941 0.715 0.584 157.5 0.978 0.975 0.972 0.966 0.976 0.981 0.965 0.945 0.718 0.584 159.0 0.979 0.978 0.972 0.967 0.977 0.981 0.964 0.946 0.716 0.581 160.5 0.981 0.977 0.972 0.966 0.979 0.982 0.961 0.947 0.722 0.589 162.0 0.982 0.978 0.978 0.965 0.977 0.981 0.962 0.950 0.720 0.577 163.5 0.982 0.979 0.980 0.970 0.976 0.982 0.959 0.953 0.724 0.579 165.0 0.984 0.981 0.978 0.971 0.979 0.984 0.963 0.955 0.726 0.577 166.5 0.986 0.979 0.978 0.968 0.980 0.982 0.964 0.954 0.723 0.577 168.0 0.984 0.981 0.981 0.970 0.977 0.981 0.962 0.956 0.730 0.578 169.5 0.985 0.979 0.980 0.969 0.981 0.985 0.963 0.955 0.729 0.584 171.0 0.987 0.981 0.980 0.968 0.981 0.982 0.962 0.956 0.732 0.579 172.5 0.988 0.983 0.979 0.968 0.980 0.985 0.966 0.959 0.739 0.595 174.0 0.988 0.982 0.980 0.969 0.980 0.986 0.967 0.958 0.734 0.577 175.5 0.990 0.981 0.981 0.970 0.982 0.986 0.965 0.957 0.738 0.579 177.0 0.990 0.981 0.985 0.972 0.982 0.986 0.966 0.960 0.740 0.589 178.5 0.988 0.984 0.983 0.973 0.987 0.988 0.969 0.959 0.742 0.577 180.0 0.990 0.985 0.984 0.970 0.983 0.987 0.968 0.963 0.745 0.590 181.5 0.988 0.982 0.984 0.975 0.986 0.988 0.972 0.965 0.747 0.585 183.0 0.996 0.983 0.986 0.972 0.984 0.988 0.966 0.967 0.750 0.579 184.5 0.992 0.985 0.985 0.974 0.987 0.990 0.971 0.965 0.750 0.582 186.0 0.995 0.986 0.985 0.973 0.986 0.988 0.969 0.968 0.753 0.578 187.5 0.995 0.984 0.984 0.974 0.985 0.988 0.970 0.970 0.755 0.582 189.0 1.001 0.994 0.991 0.979 0.991 0.995 0.977 0.972 0.764 0.579 190.5 0.993 0.987 0.985 0.975 0.986 0.990 0.974 0.972 0.759 0.588 192.0 0.992 0.986 0.990 0.975 0.987 0.986 0.972 0.972 0.761 0.575 193.5 0.997 0.985 0.989 0.976 0.985 0.990 0.972 0.971 0.764 0.586 195.0 0.995 0.987 0.990 0.980 0.986 0.989 0.970 0.973 0.765 0.571 196.5 0.995 0.990 0.988 0.975 0.986 0.990 0.972 0.977 0.765 0.588 198.0 0.996 0.989 0.990 0.976 0.989 0.990 0.974 0.976 0.771 0.583 199.5 0.995 0.988 0.988 0.977 0.988 0.991 0.973 0.977 0.770 0.589 201.0 0.994 0.988 0.988 0.978 0.986 0.989 0.972 0.978 0.773 0.586 202.5 0.999 0.990 0.995 0.987 0.989 0.988 0.974 0.974 0.776 0.577 204.0 0.998 0.992 0.988 0.977 0.990 0.993 0.975 0.979 0.774 0.576 205.5 0.998 0.991 0.991 0.978 0.989 0.992 0.975 0.980 0.778 0.590 207.0 0.999 0.987 0.995 0.980 0.988 0.989 0.972 0.980 0.779 0.587 208.5 0.999 0.990 0.992 0.978 0.990 0.989 0.976 0.979 0.780 0.581 210.0 1.001 0.987 0.994 0.981 0.985 0.988 0.974 0.980 0.784 0.573 211.5 0.998 0.991 0.991 0.980 0.990 0.994 0.977 0.986 0.785 0.583 213.0 0.999 0.989 0.994 0.981 0.989 0.993 0.976 0.984 0.790 0.579 214.5 1.003 0.991 0.995 0.983 0.992 0.992 0.977 0.986 0.791 0.587 216.0 1.003 0.989 0.997 0.982 0.990 0.990 0.977 0.986 0.793 0.578 217.5 1.000 0.992 0.997 0.981 0.990 0.994 0.978 0.987 0.795 0.579 219.0 0.998 0.991 0.994 0.983 0.995 0.997 0.982 0.987 0.798 0.588 220.5 1.001 0.994 0.993 0.980 0.994 0.995 0.979 0.987 0.797 0.580 222.0 1.001 0.993 0.998 0.984 0.991 0.993 0.979 0.988 0.802 0.577 223.5 1.000 0.995 0.995 0.982 0.992 0.995 0.982 0.990 0.806 0.581 225.0 1.004 0.994 0.995 0.983 0.993 0.993 0.982 0.992 0.804 0.578 226.5 1.003 0.996 0.993 0.984 0.994 0.996 0.983 0.993 0.804 0.581 228.0 1.002 0.992 0.995 0.985 0.995 0.996 0.983 0.992 0.807 0.589 229.5 1.004 0.994 0.999 0.984 0.993 0.995 0.981 0.993 0.811 0.575 231.0 1.005 0.995 1.001 0.986 0.994 0.994 0.982 0.993 0.812 0.581 232.5 1.004 0.996 0.995 0.984 0.995 0.998 0.980 0.996 0.816 0.581 234.0 1.005 0.994 0.997 0.985 0.993 0.995 0.984 0.994 0.815 0.586 235.5 1.006 0.995 0.997 0.985 0.993 0.995 0.983 0.995 0.819 0.581 237.0 1.003 0.995 0.997 0.986 0.997 0.999 0.987 0.998 0.820 0.582 238.5 1.006 0.996 0.996 0.986 0.995 0.998 0.986 1.002 0.822 0.577 240.0 1.005 0.994 1.002 0.988 0.997 0.994 0.985 0.993 0.828 0.579 241.5 1.009 0.995 1.001 0.987 0.995 0.997 0.984 0.999 0.828 0.588 243.0 1.010 0.997 1.001 0.987 0.996 0.996 0.985 0.997 0.829 0.582 244.5 1.008 0.996 1.001 0.988 0.997 0.997 0.987 1.001 0.829 0.578 246.0 1.009 0.999 1.002 0.988 0.996 0.998 0.988 1.004 0.829 0.580 247.5 1.009 0.998 1.001 0.989 0.996 0.998 0.987 1.003 0.835 0.579 249.0 1.011 0.998 1.004 0.990 0.997 0.998 0.985 1.004 0.835 0.584 250.5 1.011 0.998 1.004 0.987 0.997 0.999 0.988 1.006 0.839 0.582 252.0 1.010 1.000 1.000 0.989 0.997 1.001 0.990 1.009 0.836 0.589 253.5 1.008 0.996 1.007 0.995 1.000 0.999 0.989 1.006 0.843 0.576 255.0 1.010 0.998 1.002 0.989 0.998 1.001 0.993 1.012 0.844 0.587 256.5 1.011 0.999 1.006 0.991 0.996 0.997 0.988 1.006 0.845 0.574 258.0 1.008 1.001 1.003 0.990 0.999 1.000 0.993 1.014 0.844 0.580 259.5 1.010 1.001 1.003 0.989 1.000 1.001 0.992 1.016 0.848 0.578 261.0 1.012 1.000 1.003 0.989 0.998 1.000 0.993 1.016 0.851 0.583 262.5 1.010 0.999 1.007 0.996 0.999 0.999 0.991 1.012 0.852 0.573 264.0 1.012 1.001 1.007 0.991 0.999 1.001 0.993 1.017 0.858 0.582 265.5 1.012 1.001 1.003 0.989 1.001 1.001 0.993 1.021 0.854 0.586 267.0 1.013 1.001 1.005 0.992 0.999 1.001 0.994 1.020 0.857 0.586 268.5 1.009 0.997 1.006 0.992 1.002 1.004 0.997 1.026 0.858 0.579 270.0 1.014 0.999 1.009 0.992 0.997 1.000 0.995 1.016 0.861 0.578 271.5 1.015 1.000 1.008 0.996 1.000 1.001 0.995 1.023 0.862 0.581